The harmful effects of fungi insorghum silage are an important matter inmany countries with hot climates. This cultureis very susceptible to fungal contaminationand both the storage and processing ofthis substrate are ideal for the developmentof fungi there are able to produce mycotoxins.The contamination with mycotoxins,secondary products of the metabolism ofcertain fungi, that promotes risks to bothanimal and human health, causes numerouslosses for the country. The aim of this study was to identify the toxicogenic fungi present in sorghum silage for beef cattle consumption and determine the occurrence of aflatoxin B1 (AFB1) in the same substrate. A total of 60 pre-fermented and 60 post-fermented samples of sorghum silage were analyzed. Total fungal counts and natural incidence of toxigenic Aspergillus, Penicillium y Fusarium species were performed on dichloranrose bengal chloranphenicol agar, dichloranglycerol agar 18 % and Nash-Snyder culture media. Aflatoxin B1 contamination was determined using high pressure liquid chromatography (HPLC). About 30 % of samples from pre-fermented sorghum and 55 % of post-fermented samples were above there commended limits (1.0 x 104 CFU g-1). The most frequent fungal species in both types of sorghum samples were A. flavus, P. citrinum,P. islandicum and F. verticillioides. Anaverage of 32 % samples, pre and post-fermented,were positive for AFB1. The presence of fungi and AFB1 in the feedstuffs indicates contamination. This toxin could affect animal productivity and health. This fact requires periodic monitoring to prevent the occurrence of mycotoxicosis in animal production.
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