Resumen
Las orquídeas son un grupo de plantas muy diverso y de gran importancia ornamental, ecológica y económica; comprender su biología es esencial para su conservación y mejoramiento. Este estudio desarrolló un protocolo eficiente para obtener y teñir células en metafase en tres especies de orquídeas, utilizando técnicas citogenéticas clásicas como el pretratamiento de raíces, fijación y tinción para visualizar cromosomas. Los resultados mostraron células con cromosomas bien dispersos y contrastados, permitiendo identificar diferencias en número y morfología entre especies. El protocolo optimizado resulta útil para estudios cromosómicos y para apoyar programas de reproducción y mejoramiento genético.
Citas
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