Abstract
The aquaculture industry has a high growth with a projection of reaching 106 million tons in 2030 worldwide. For this, the implementation of genetic management and selection programs, based on the monitoring of the genetic diversity, inbreeding and pedigree of the cultivated stocks, are needed. In this study, a genetic analysis platform, known as 2bRAD, was developed to genetically characterize aquaculture species by means of low-density SNP (Single Nucleotide Polimorphisms) panels of 100-500 genetic markers. The 2bRAD technique, implemented with the enzyme BcgI and using four selective bases in the adaptors, resulted in low-density panels of 114 and 159 SNPs for the Pacific oyster Crassostrea gigas and almaco jack Seriola rivoliana, respectively. These panels were validated with parentage and paternity assignment tests, and thus, they are suitable to study genetic diversity and pedigree follow-up in cultivated stocks. The panel obtained in the whiteleg shrimp Penaeus (Litopenaeus) vannamei was of medium-density (2874 SNPs), and thus, has other type of applications. The developed 2bRAD platform is potentially applicable to other marine fish species such as red snapper, rose spotted snapper and totoaba.
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