Abstract
Tomato brown rugose fruit virus (ToBRFV) has recently emerged as an emerging threat and represents a considerable economic risk in tomato production. The objective of this work was to isolate ToBRFV from three tomato varieties located in Coahuila, Mexico. The purpose was to evaluate the pathogenicity of ToBRFV in tomato plants and establish a diagrammatic scale that permits an accurate visualization of the severity of symptoms in leaflets and fruit. The FQ3 isolate had a significant impact on the agronomic variables of tomato plants, through a 53.9 % decrease in plant height and 38.9 % in the dry weight of the aerial part of the plant. Regarding quality variables, a 43.1 % reduction in fruit equatorial diameter and 43.2 % in fruit yield resulted. A diagrammatic scale application allowed a more precise quantification of the severity caused by the various isolates of ToBRFV. Isolate FQ3 generated an incidence ranging from 33.9 to 50 % and a severity ranging from 41 to 87.5 % more than other isolates. These findings demonstrate the importance of understanding and managing the variability in responding tomato plants to different isolates of ToBRFV, which may have significant consequences for the production of tomatoes affected by this disease.
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