Abstract
The ornamental relevance of orchid species lies in their morphological characteristics. Notylia barkeri Lindl. It is listed in Appendix II of the Convention on International Trade in Endangered Species of Wild Fauna and Flora. Therefore, it is imperative to institute preservation methods to save its long-term viability. The objective was to establish an in vitro conservation protocol for N. barkeri. Different concentrations of abscisic acid (0.1 and 2 mg L-1) and ancimidol (0.1 and 2 mg L-1) were evaluated in Murashige and Skoog medium. After 180 days, the percentage of survival, number and length of shoots, number of leaves, number and length of roots were evaluated. The highest survival percentage (85.71%) is monitored in control treatment. While in 1 mg L-1 of abscisic acid, 57.14% was observed. The lowest percentages were observed in ancimidol. It will be observed that 1 mg L-1 of abscisic acid will reduce the length of the shoots without affecting their survival unlike when ancimidol is used. Furthermore, 1 mg L-1 of abscisic acid reduces the number and length of roots. Our results may contribute to the conservation of this species with ornamental interest.
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