Abstract
In vitro shoot proliferation of Vaccinium corymbosum var. Biloxi, was evaluated in culture media that varied in inorganic salts and pH levels. Stem segments 2 cm long that had only axillary buds and other segments had apical and axillary buds were established on culture media with inorganic salts WPM50% (Woody Plant Medium), or MS50% (Murashige and Skoog) or the combination MS50%-WPM50%, at different pH levels (4. 5, 5.0 and 5.5), and contained 25 g L-1 sucrose, 100 mg L-1 Myo-inositol, 0.4 mg L-1 thiamine-HCL, 2 mg L-1 2iP, 0.5 mg L-1 pyridoxine, 0.5 mg L-1 nicotinic acid, 2 mg L-1 glycine, 5.7 g L-1 agar. The variables shoot length, number of leaves, and number of shoots were evaluated at 40, 80 and 120 days of incubation. The experiment was established according to a completely randomized design, with a 3×3×2 factorial arrangement. The stem segments established in culture media with inorganic salts MS50%-WPM50% with pH levels 4.5 or 5.0 developed axillary shoots that were larger in size (5.5 and 5.8 cm) and number of leaves (11.5 and 11.8).
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