Abstract
Persian lime (Citrus x latifolia Tan.) is a key crop in Veracruz, with production affected by fungal diseases. To reduce the use of chemical fungicides, the use of Biological Control Agents (BCAs), such as bacteria from the Bacillus genus, has been investigated. In this study, the in vitro antagonistic effect of Bacillus velezensis (Bv) and two strains of Bacillus subtilis (Bs1 and Bs2) was evaluated against the pathogens responsible for dieback (Lasiodiplodia spp.) and anthracnose (Colletotrichum sp.), isolated from Persian lime orchards in Tierra Blanca, Veracruz. The Percentage of Radial Growth Inhibition (PRGI) was measured on potato dextrose agar plates.
Bacillus subtilis strains showed inhibition of the evaluated pathogens. Bs2 presented the highest inhibition percentages (66.69% and 61.78%) against Lasiodiplodia spp. (Las1 and Las2). Bs1 showed 49.7% inhibition against Colletotrichum sp. (Colle). In contrast, Bacillus velezensis (Bv) exhibited a low antagonistic effect, with only 8.4% inhibition against Las1 and no inhibition against the other strains. These results indicate that Bacillus subtilis Bs1 and Bs2 strains have high potential as BCAs against pathogens affecting Persian lime production.
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