Abstract
The Lilium sp., or lily, is a plant of significant ecological, cultural, and commercial importance, valued in gardening, fragrances, and traditional medicine. Given the limitations of its natural reproduction, in vitro propagation is essential for its efficient and sustainable cultivation, producing disease-free plants with high genetic quality. A major challenge of this technique is contamination, which requires strict disinfection protocols. In this context, silver nanoparticles (AgNPs) stand out for their antimicrobial properties, reducing contamination and improving process efficiency, while also offering a sustainable alternative to harsh chemical treatments. In this study, AgNPs Argovit™ were evaluated at concentrations of 0, 25, 50, and 100 mg•L⁻¹ with immersion times of 5, 10, and 15 minutes. The best asepsis (94%) was achieved with 100 mg•L⁻¹ and 15 minutes of immersion, while the highest sprouting (7.03 microbulbs per explant) occurred with 25 mg•L⁻¹ and the same duration. AgNPs also reduced phenolic oxidation in explants, optimizing the in vitro propagation of Lilium sp. These findings highlight their potential for the commercial production and conservation of this ornamental species.
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