In vitro propagation of Pinguicula moranensis H.B.K., var. Neovolcánica Z.

Abstract

Seeds of Pinguicula moranensis were collected in the "El Chico" National Park in the state of Hidalgo, México, in order to develop a propagation strategy for in vitro mass multiplication. Seeds were germinated in medium ¼ MS supplemented with 100 mg L^-1 inositol, 0.4 mg L^-1 Thiamine, 20 g L^-1 sucrose, 8.5 g L^-1 of agar, pH 5.7 (Basal medium with organics, MBO), without growth regulators showing low germination (5 %). Seedlings of 10 days, were incubated in medium MBO with BA (0, 0.5 mg L^-1) in combination with NAA (0, 0.5 mg L^-1), or 2,4D (0, 1.0 mg L^-1). The presence of 2,4-D (1.0 mg L^-1), caused necrosis in this species, however MBO medium, supplemented with  BA 0.5 mg L^-1 and NAA 0.5 mg L^-1 , allowed 100% induction of callus which observed defined and friable. The callus obtained was incubated in the above medium MBO with BA (0, 1 and 2 mg L^-1) and NAA (0, 0.1, 0.5 mg L^-1). The best response was observed with medium MBO supplemented with BA (1mg L^-1) and NAA (0.5 mg L^-1), finding an average of 1957 + 125 shoots per jar. The shoots formed were obtained with a large proportion of vitrification, so they were incubated in the same medium but without growth regulators, where they were devitrified and individualized, rooting spontaneously.  Allowing thereby defining an efficient protocol for mass propagation of the species.