EN PRENSA. Antioxidant and antibacterial effect of different extracts from Tithonia tubaeformis.

Abstract

The genus Tithonia has been extensively studied to determine its bioactive effects of pharmaceutical and nutritional interest; therefore, the objective of this study was to determine the antioxidant and antibacterial effect of five extracts of Tithonia tubaeformis, obtained with ascending polarity solvents, hexane, dichloromethane,
ethyl acetate, methanol, and ethanol. In addition, tannins, alkaloids, and total phenols were identified. The antioxidant effect of ABTS radicals, DPPH, and ferric ion reducing power was measured, and the Minimum Inhibitory Concentration of each extract was determined on Escherichia coli, Salmonella typhimurium, Salmonella enteritidis, and Listeria monocytogenes. The results showed that the five extracts have antioxidant
effects on ABTS, DPPH radicals, and ferric ion-reducing potential; however, methanol and ethanol had a statistically significant effect (p < 0.05). In addition, methanol, ethanol, and ethyl acetate extracts showed antimicrobial activity; ethanol solvent was the best, with a Minimum Inhibitory Concentration of 1 mg/mL on Escherichia coli and 3 mg/mL on Salmonella typhimurium, Salmonella enteritidis, Escherichia coli, and
Listeria monocytogenes respectively. These results suggest that Tithonia tubaeformis may be of pharmaceutical and nutritional interest due to the bioactive effects of phytochemicals in its extracts.