Herpesvirus de los ostreidos tipo 1 en la almeja mano de león Nodipecten subnodosus de Baja California Sur, México

Abstract

This study is the first report of the presence of Ostreid herpes virus 1 (OsHV-1) in the scallop Nodipecten subnodosus from Baja California Sur, Mexico; collected from 2010 to 2012.The retrospective study was conducted to evaluate whether OsHV-1 was implicated in the population decline of 2010. The virus was detected by PCR, and viral particles were quantified by qPCR. The OsHV-1 detection was conducted by PCR amplification of three different regions of the genome used to identify OsHV-1 variants. A phylogenetic analysis indicated the phylogenetic position of N. subnodusus OsHV-1 among OsHV-1 samples previously collected in different countries and bivalves. Phylogeny analyses indicated differences between OsHV-1 of N. subnodosus and OsHV-1 μVar. Similarity analyses showed differences between the OsHV-1 of Crassostrea gigas and the OsHV-1 of N. subnodosus from Mexico, however the sequence was identical to that of OsHV-1 from France 1993. This study contributes to better understand the genetic diversity of OsHV-1 and to identify potential host of the virus or species involved in the viral cycle.