Abstract
Moringa oleifera is a species with a wide global distribution due to its adaptive capacity and nutritional properties. In Mexico, there have been several reintroductions of this species and although morphological diversity has been observed, the extent of its diversity at the DNA level is unknown. The objective was to determine the diversity and genetic structure of 14 populations of M. oleifera cultivated in Mexico through SNP. The seeds were germinated in a greenhouse and DNA was extracted from young leaves and stems using a CTAB-based protocol. This DNA was used to genotype each population with DArTseqTM technology. 9,862 SNPs were obtained, of which 0.64% were polymorphic. Moderate levels of diversity were observed within the populations (Ae=1.52, He=0.33, Ho= 0.44, Shannon index = 0.44). The genetic background of the populations allowed two groups to be visualized through cluster analysis and PCoA. The genetic structure of the populations using STRUCTURE indicated that the ancestry comes from two original populations (K = 2). The 14 moringa populations are diverse at the high-quality DNA level for accurate and reliable sequencing.
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