Keywords
Orchid, special protection, in vitro culturing, molecular markers.
in vitro culture
Molecular markers
How to Cite
Métricas de PLUMX
Abstract
Laelia speciosa (Kunth) Schltr., is an epiphytic orchid endemic to Mexico, naturally found in cold, dry mountainous regions at elevations between 1,400 and 2,400 meters above sea level. It is protected under NOM-059-SEMARNAT-2010 and is subject to international trade restrictions due to illegal harvesting and habitat destruction. This study aimed to develop alternative propagation methods and perform molecular characterization of L. speciosa using in vitro techniques and molecular markers. Different conditions for seed germination, shoot multiplication, and acclimatization were tested. Molecular analysis was conducted using RAPD markers with 16 a-series primers. The highest germination rates were achieved with the addition of benzyladenine, WS vitamins, and malt extract. A combination of 2,4-D and kinetin promoted optimal explant development, while a substrate composed of red tezontle and peat supported successful acclimatization. Molecular characterization with RAPD markers revealed genetic variation both within and between species. These findings emphasize the importance of species-specific in vitro protocols, combined with molecular tools, for conserving and propagating this endemic orchid.
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