Keywords
histology
darkness
thidiazuron
How to Cite
Métricas de PLUMX
Abstract
The induction of protocorm-like bodies (PLBs) in Cymbidium 'King Arthur' hybrid was evaluated using leaf segments and shoot tips in vitro. Different concentrations (0, 0.45, 2.27, and 4.54 μM) of thidiazuron (TDZ) were studied under photoperiod (16 h light/8 h dark) and darkness for four months. Survival (%) and the total number of PLBs formed were determined. Induction was analyzed histologically. Shoot tips only formed PLBs. A maximum of 30 and 67 PLBs were obtained with 4.54 μM TDZ under photoperiod and darkness, respectively. Histological analysis confirmed formation via direct organogenesis as a potential alternative for producing multiple shoots.
References
Cuervo-Andrade, J. L., Gutiérrez-Rodríguez, G. A., Albarracin-Bohórquez, N., Sánchez-Sánchez, J. M., Escobar-Pardo, O. F., Flautero-Murillo, C. L., Mancera-Rodríguez, L. P., Beltrán-Zapata, G. D., & Pacheco-Salamanca, R. A. (2022). Generalidades del cultivo Cymbidium. Corporación Unificada Nacional de Educación Superior CUN.
https://repositorio.cun.edu.co/handle/cun/3582
Balilashaki, K., Martinez-Montero, M. E., Vahedi, M., Cardoso, J. C., Silva Agurto, C. L., Leiva-Mora, M., Feizi, A., & Musharof Hossain, M. (2023). Medicinal use, flower trade, preservation and mass propagation techniques of Cymbidium orchids-An overview. Horticulturae, 9(6), 690. https://doi.org/10.3390/horticulturae9060690
Bhowmik, T. K., & Rahman, M. M. (2017). Effect of different basal media and PGRs on in vitro seed germination and seedling development of medicinally important orchid Cymbidium aloifolium (L.) Sw. Journal of Pharmacognosy and Phytochemistry, 6, 167-172.
Campol, J. R., Naing, A. H., Aung, H. M., Cho, S. B., Kang, H., Chung, M. Y., & Kim, C. K. (2024). Production of genetically stable and Odontoglossum ringspot virus-free Cymbidium orchid ‘New True’plants via meristem-derived protocorm-like body (PLB) subcultures. Plant Methods, 20(1), 145. https://doi.org/10.1186/s13007-024-01269-1
Cardoso, J. C., Zanello, C. A., & Chen, J. T. (2020). An overview of orchid protocorm-like bodies: Mass propagation, biotechnology, molecular aspects, and breeding. International Journal of Molecular Sciences, 21(3), 985. https://doi.org/10.3390/ijms21030985
De, L. C. (2022). Good Agricultural Practices of Cymbidium Orchids. Vigyan Varta, 3(3), 44-56.
Fritsche, Y., Sanches-Ornellas, T., Stefenon, V. M., & Guerra, M. P. (2022). Ploidy mosaics: does endopolyploidy in explants affect the cytogenetic stability of orchids regenerated from PLBs?. Plant Cell, Tissue and Organ Culture, 149(3), 697-713. https://doi.org/10.1007/s11240-022-02238-z
Gow, W. P., Chen, J. T., & Chang, W. C. (2009). Effects of genotype, light regime, explant position and orientation on direct somatic embryogenesis from leaf explants of Phalaenopsis orchids. Acta Physiologiae Plantarum, 31(2), 363-369. https://doi.org/10.1007/s11738-008-0243-6
Julkifle, A. L., Poobathy, R., Samian, R., & Subramaniam, S. (2012). Histological analyses of PLBs of Dendrobium sonia-28 in the recognition of cell competence for regeneration and Agrobacterium infection. Plant Omics, 5(6), 514-517. https://www.pomics.com/subaramanian_5_6_2012_514_517.pdf
Kaewubon, P., Sangdam, S., Thammasiri, K., & Meesawat, U. (2010). Plant regeneration through somatic embryogenesis from callus-derived PLBs of tropical slipper orchid (Paphiopedilum niveum (Rchb. f.) Pfitz.). Floriculture and Ornamental Biotechnology, 4(1), 29-35.
Kruglova, N., Zinatullina, A., & Yegorova, N. (2023). Histological approach to the study of morphogenesis in callus cultures in vitro: a review. International Journal of Plant Biology, 14(2), 533-545. https://doi.org/10.3390/ijpb14020042
Mayer, J. L. S., Stancato, G. C., & Appezzato-Da-Glória, B. (2010). Direct regeneration of protocorm-like bodies (PLBs) from leaf apices of Oncidium flexuosum Sims (Orchidaceae). Plant Cell, Tissue and Organ Culture, 103(3), 411-416. https://doi.org/10.1007/s11240-010-9782-9
Mengxi, L., Zhigang, X., Yang, Y., & Yijie, F. (2011). Effects of different spectral lights on Oncidium PLBs induction, proliferation, and plant regeneration. Plant Cell Tissue Organ Culture, 106, 1–10 https://doi.org/10.1007/s11240-010-9887-1
Murashige, T., & Skoog, F. (1962). A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiologia plantarum, 15(3). https://doi.org/10.1111/j.1399-3054.1962.tb08052.x
Naderi Boldaji, H., Dianati Daylami, S., & Vahdati, K. (2023). Use of light spectra for efficient production of PLBs in temperate terrestrial orchids. Horticulturae, 9(9), 1007. https://doi.org/10.3390/horticulturae9091007
Pant, B. (2013). Medicinal orchids and their uses: Tissue culture a potential alternative for conservation. African Journal of plant science, 7(10), 448-467. https://doi.org/10.5897/ajps2013.1031
Park, J., Lee, H. B., An, S. K., Lee, J. H., & Kim, K. S. (2021). Increasing duration and intensity of nighttime supplemental lighting promotes growth and photosynthesis in young Cymbidium plants. Horticulture, Environment, and Biotechnology, 62(5), 679-690. https://doi.org/10.1007/s13580-021-00352-z
So Young, P., Murthy, H. N., & Kee Yoeup, P. (2000). Mass multiplication of protocorm-like bodies using bioreactor system and subsequent plant regeneration in Phalaenopsis. Plant Cell, Tissue and Organ Culture, 63(1), 67-72. https://doi.org/10.1023/A:1006420116883
Park, S. Y. E. C., Yeung, E., Chakrabarty, D., & Paek, K. (2002). An efficient direct induction of protocorm-like bodies from leaf subepidermal cells of Doritaenopsis hybrid using thin-section culture. Plant Cell Reports, 21(1), 46-51. https://doi.org/10.1007/s00299-002-0480-x
Pyati, A. N. (2023). In vitro Regeneration of an Endangered Medicinal Orchid Dendrobium crepidatum Lindl. & Paxton through Protocorm like bodies. Plant Tissue Culture and Biotechnology, 33(1), 1-8. https://doi.org/10.3329/ptcb.v33i1.66753
Retheesh, S. T., & Bhat, A. I. (2011). Genetic transformation and regeneration of transgenic plants from protocorm-like bodies of vanilla (Vanilla planifolia Andrews) using Agrobacterium tumefaciens. Journal of Plant Biochemistry and Biotechnology, 20(2), 262-269. https://doi.org/10.1007/s13562-011-0057-2
Royal Horticultural Society. (2025, diciembre 30). International Orchid Register. https://apps.rhs.org.uk/horticulturaldatabase/orchidregister/orchidregister.asp
Teixeira da Silva, J. A. (2013). Impact of paper bridges, activated charcoal, and antioxidants on growth and development of protocorm-like bodies of hybrid Cymbidium. In Vitro Cellular & Developmental Biology-Plant, 49(4), 414-420. https://doi.org/10.1007/s11627-013-9532-x
Teixeira da Silva, J. A., Cardoso, J. C., Dobránszki, J., & Zeng, S. (2015). Dendrobium micropropagation: a review. Plant cell reports, 34(5), 671-704. https://doi.org/10.1007/s00299-015-1754-4
Valley P. (1976). JB-4 embedding kit. Polysciences Inc. Warrington. Printed in U.S.A. 1-3.
Zhao, P., Wang, W., Feng, F.S., Feiwu, Yang, Z. & Wang, J. W. (2007). High-frequency shoot regeneration through transverse thin cell layer culture in Dendrobium Candidum Wall Ex Lindl. Plant Cell Tiss Organ Cult, 90, 131–139. https://doi.org/10.1007/s11240-006-9181-4
Zhou, Y., Yang, Y., He, S., Xing, X., Qu, Y., & Xu, Y. (2025). Highly Efficient Agrobacterium-mediated Stable Transformation of Cymbidium goeringii. HortScience, 60(7), 1026-1031. 10.21273/HORTSCI18574-25
This work is licensed under a Creative Commons Attribution 4.0 International License.
Copyright (c) 2026 Revista Bio Ciencias