Abstract
Bovine neosporosis negatively affects reproductive outcomes and has negative economic impacts. The objective was to study neosporosis prevalence in cows and bulls, and sperm quality in bulls positive for N. caninum, in the municipalities of Ometepec and Cuajinicuilapa in the Costa Chica region of the state of Guerrero, Mexico. At 38 farms, samples were collected from 300 cows and 100 bulls of reproductive age. Blood samples (10 mL) were taken from the coccygeal vein. These were centrifuged (3500 rpm 15 min-1) to separate the plasma, which was stored in 1.5 mL Eppendorf tubes at -20 °C until analysis. Neospora caninum antibodies were identified with an ID Screen® Neospora caninum Competition kit for ELISA. Evaluated sperm variables were individual motility, concentration and morphology. Of the 286 samples from cows, 50 were positive for N. caninum (17.4% prevalence). Of the 92 samples evaluated from bulls, eight were positive (8.7% prevalence), the general prevalence was 15.34%. Sperm concentration was lower in bulls positive for N. caninum. In the sampled population, neosporosis affects sperm quality in infected bulls, and is more prevalent in cows than in bulls.
References
Ascencio-Díaz, P., Núñez-Martínez, G., Sollano-Mendieta, C., Rojas-García, R., & Bottini-Luzardo, M. (2020). Primer reporte de Neospora caninum en el ganado bovino de la Costa Chica de Guerrero, México. Agroproductividad, 13, 87-89. https://doi.org/10.32854/agrop.vf.1641
Bahrami, S., Reza, A., Gooraninejad, S., & Bakrayi, O. (2018). Effect of natural neosporosis on levels of testosterone and thyroid hormone bulls. Journal of the Hellenic Veterinary Medical Society, 69, 984-990. https://doi.org/10.12681/jhvms.18028
Bahrami, S., Mousavi, S. S., Alborzi, A. R., & Ghodratollah, M. (2019). Sperm quality and hormonal levels in C57BL/6 mice infected with Neospora caninum. Veterinary Parasitology, 273(1), 5-10. https://doi.org/10.1016/j.vetpar.2019.07.010
Periódico Oficial del Estado de Guerrero. (2023, 06 de agosto). https://periodicooficial.guerrero.gob.mx/wp-content/uploads/2023/04/P.O-32-ALCANCE-II-21-ABRIL-2023.pdf
Cantón, G., Katzer, F., Maley, S., Bartley, P., Benavides-Silván, J., Palarea-Albaladejo, J., Pang, Y., Smith, S., Rocchi, M., Buxton, D., Innes, E., & Chianini, F. (2014). Inflammatory infiltrarion into placentas of Neospora caninum challeged cattle correlates with clinical outcome of pregnancy. Veterinary Research, 45(1), 11. https://doi.org/10.1186/1297-9716-45-11
García-Vazquez. Z., Rosario-Cruz, R., Ramos-Aragon, A., Cruz-Vazquez, C., & Mapes-Sanchez, G. (2005). Neospora caninum seropositivity and association with abortions in dairy cows in Mexico. Veterinary Parasitology, 134(1-2), 61-5. https://doi.org/10.1016/j.vetpar.2005.07.007
García-Vázquez, Z., Rosario-Cruz, R., Mejía-Estrada, F., Rodríguez-Vivas, I., Romero-Salas, D., Fernández-Ruvalcaba, M., & Cruz-Vázquez, C. (2009). Seroprevalence of Neospora caninum antibodies in beef cattle in three southern states of Mexico. Tropical Animal Health and Production, 41, 749-753. https://doi.org/10.1007/s11250-008-9247-x
Gharekhani, J., Mohammed, R., Heidari, R., Hajipour, N., Trotta, M., & Villanueva-Saz, S. (2023). Assessment of Neospora caninum infection in bulls using serological and molecular techniques. Veterinary Parasitology: Regional Studies and Reports, 46, 100940. https://doi.org/10.1016/j.vprsr.2023.100940
Gondim, L. F. P., McAllister, M. M., Mateus-Pinilla, N. E., Pitt, W. C., Mech, L. D., & Nelson, M. E. (2004). Transmission of Neospora caninum between wild and domestic animals. Journal of Parasitology, 90, 1361:1365. https://doi.org/10.1645/GE-341R
Jafari, R., Asadpour, R, Nematolahi, A., & Hosseinninejad, M. (2012). Detection of Non-spermatozoal cells of Neospora caninum in fresh semen of naturally infected bulls. Acta Scientiae Veterinariae, 40(2), 1-7. https://www.redalyc.org/articulo.oa?id=289023567008
Kemel, C., Salamone, M., Van Loo, H., Latour, C., Vandeputte, S., Callens, J., Hostens, M., & Opsomer, G. (2022) Unaffected semen quality parameters in Neospora caninum seropositive Belgian Blue bulls. Theriogenology, 191, 10-15. https://doi.org/10.1016/j.theriogenology.2022.07.013
Koziol, J., & Armstrong, C. (2018) Evaluation of Semen Quality. In: J. Koziol & C. Armstrong (eds), Manual for Breeding Soundness Examination of Bulls (pp. 83-104). Society for Theriogenology.
Marugan-Hernandez, V. (2017). Neospora caninum and bovine neosporosis: Current vaccine research. Journal of Comparative Pathology, 157,193-200. https://doi.org/10.1016/j.jcpa.2017.08.001
Medina, L., Cruz-Vázquez, C., Quezada, T., Morales, E. & García-Vázquez, Z. (2006). Survey of Neospora caninum infection by nested PCR in aborted fetuses from dairy farms in Aguascalientes, Mexico. Veterinary Parasitology, 136, 187-191. https://doi.org/10.1016/j.vetpar.2005.11.003
Momont, H., & Checura, C. (2015). Ultrasound Evaluation of the Reproductive tract of the Bull. In: R. Hopper (ed), Bovine Reproduction, (pp. 79-91). Wiley Blac.
Mondragón-Zavala, K., Cruz-Vázquez, C., Medina-Esparza, L., Ramos-Parra, M., & García-Vázquez, Z. (2011). Neospora caninum infection in beef cattle reared under grazing conditions in north-central Mexico. Revista MVZ Córdoba, 16(2), 2484-2490. https://revistamvz.unicordoba.edu.co/article/view/1007
Montiel-Peña, T., Romero-Salas, D., García-Vázquez, Z., Medina-Esparza, L., & Cruz-Vázquez, C. (2011). Neosporosis bovina en ranchos ganaderos de la zona norte del estado de Veracruz, México. Tropical and Subtropical Agroecosystems, 13(3), 469-479. https://www.revista.ccba.uady.mx/ojs/index.php/TSA/article/view/1387
Ojeda-Carrasco, J. J., Espinosa-Ayala, E., Hernández-García, P. A. Rojas-Martínez, C., & Álvarez-Martínez, J. A. (2016). Seroprevalencia de enfermedades que afectan la reproducción de bovinos para leche con énfasis en neosporosis. Ecosistemas y recursos agropecuarios, 3(8), 243-249. https://doi.org/10.19136/era.a3n8.755
Pereyra, W., R., Suarez, V. R., Cardoso, N., Gual, I., Martínez, G. M., Capozzo, A. V., & Mansilla, F. C. (2021). Prevalencia sérica de Neospora caninum y factores de riesgo asociados a su transmisión en tambos de la provincia de Salta, Argentina. Revista Argentina de Microbiologia, 53(2), 51-60. https://doi.org/10.1016/j.ram.220.06.01
Polo, C., García-Seco, T., Díez-Guerrier, A., Briones, V., Domínguez, L., & Pérez-Sancho, M. (2023). What about the bull? A systematic review about the role of males in bovine infectious infertility within cattle herds. Veterinary and Animal Science, 19, 100284. https://doi.org/10.1016/j.vas.2023.100284
Pulido, M. O., Díaz, A. M., & Andrade, R. J. (2017). Asociación entre variables reproductivas y anticuerpos anti Neospora caninum en bovinos lecheros de un municipio de Colombia. Revista Mexicana de Ciencias Pecuarias, 8(2), 167-174. https://doi.org/10.22319/rmcp.v8i2.4439
Reichel, M., Ayanegui-Alcérreca, M. A., Gondim, L. F. P., & Ellis, J. T. (2013). What is the global economic impact of Neospora caninum in cattle - The billion dollar question. International Journal for Parasitology, 43, 133-142. https://doi.org/10.1016/j.ijpara.2012.10.022
Reichel, M. P., Wahl, M. L., & Ellis, J. T. (2020). Research into Neospora caninum - What have we learnt in the last thirty years? Pathogens, 9(6), 505. https://doi.org/10.3390/pathogens9060505
Romero-Salas, D., García-Vázquez, Z., Montiel, F., Montiel-Peña, T., Aguilar-Domínguez, M., Medina-Esparza, L., & Cruz-Vázquez, C. (2010). Seroprevalence of Neospora caninum antibodies in cattle in Veracruz, Mexico. Journal of Animal and Veterinary Advances, 10, 1445-1451. https://doi.org/10.3923/javaa.2010.1445.1451
Salinas M., J. A., Mora, J. de J., Zarate, J. J., Riojas, V. M., Hernández, G., Dávalos, G., Ramírez, R., Galán, L. C., & Ávalos, R. (2005). Frecuencia de anticuerpos contra Neospora caninum en ganado bovino del noreste de México. Veterinaria México, 36(3), 303-311. https://www.redalyc.org/pdf/423/42336306.pdf
van Velsen, C. M. (2021). Neosporosis in Bulls: potential for venereal transmission, and effect on semen quality and production. New Zealand Veterinary Journal, 69(4), 193-200. https://doi.org/10.1080/00480169.2020.1854883
World Health Organization [WHO]. (2010). Examination and processing of human semen. 5th edition. https://www.who.int/publications/i/item/9789240030787
Zárate-Martínez, J. J., Rosete-Fernández, J. V., Socci-Escatell, G. A., Fragoso-Islas, A., Olazarán-Jenkins, S., Granados-Zurita, L., & Ríos-Utrera, A. (2021). Prevalencia de anticuerpos séricos bovinos contra Neospora caninum en las zonas Central y Sur del Golfo de México. Revista MVZ Córdoba, 26(1), e1996. https://doi.org/10.21897/rmvz.1996
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